Areas

Health

Fields of use

Antibody affinity purification, Antibody immobilization

Current state of technology

Prototype

Intellectual property

PCT/EP2019/052484

Developed by

University of Ljubljana, Faculty of Pharmacy

Reference

821-3/2018

Contact

Knowledge Transfer Office UL


Gabriela Droga Mazovec
Tel: +386 70 538 284
E-mail: gospodarstvo@uni-lj.si

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Background

Simple linear peptides selective for the Fc region of antibodies were developed using the principles of molecular evolution. When coupled to chromatographic matrix, synthetic peptides are capable of enriching antibodies from complex feedstocks, reaching purities comparable to conventional staphylococcal protein A-based methods.

Description of invention

In the proof-of-principle experiments the peptides were shown to possess affinity to selectively enrich antibodies from cell growth medium and human serum using peptide-functionalized pull-down beads and a prototype affinity chromatographic column. Another potential application of peptides as biorecognition reagents is in immobilizing capturing antibodies to a biosensor surface. The technology is especially appropriate for research purposes in areas of molecular biology, biophysics, as well as preclinical evaluation and development of therapeutic or diagnostic antibodies.

Main advantages

In contrast to protein A, the peptide ligands are less susceptible to harsh inter-run cleaning-in-place decontamination conditions, since they do not rely on intricate peptide chain folding for binding affinity and can be affordably produced synthetically. This translates to extended affinity column lifetime. Furthermore, the peptides bind all subclasses of immunoglobulin G.